Measurement and Testing

Adding More Power to your GC-MS Analysis through Deconvolution - Presented by Anthias Consulting Ltd at PEFTEC 2015

Jan 20 2016 Comments 0

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If you were not able to attend the PEFTEC conference in November 2015, here is an opportunity to view the presentation Diane gave on the day.

In separation techniques like gas chromatography, good chromatographic resolution is necessary to analyse sample components for both qualitative and most importantly accurate quantitative analyses. When separation techniques are hyphenated to mass spectrometry, like in GC-MS, another dimension of separation is added in the form of analytical resolution. This can reduce the necessity for good chromatographic resolution, unless components are isomers, but can also enable the separation of many more components in shorter run times. In the method development and in many cases the analysis of target compounds, significant ions can be extracted and compared to identify their retention times, frequently coeluting with other target plus matrix components. But when analysing a sample to identify unknowns, how do you know if you have chromatographically co-eluting peaks or components hidden under the baseline? This presentation looks at how deconvolution works and how powerful and useful it can be, even when just analysing target analytes in what looks like relatively clean samples.

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